摘要
本文探讨获得正常和甲亢甲状腺细胞的方法及其培养条件，研究其对ＴＳＨ和甲状腺刺激抗体（ＴＳＡｂ）的反应性，分析两种甲状腺细胞在甲状腺特异性抗体测定中的临床价值。结果表明：１．正常和甲亢两种甲状腺组织经胰酶和胶原酶消化６０～９０分钟，即可获得大量活力良好的人甲状腺细胞；２．甲状腺细胞在以Ｅａｇｌｅ营养液为基础的培养条件下，随每孔细胞数的增多，对ＴＳＨ和ＴＳＡｂ刺激生成ｃＡＭＰ的量亦增加，并且在０～９６小时的培养时间里，甲状腺细胞ｃＡＭＰ的释放值递增；３．以正常或甲亢甲状腺细胞作为靶细胞，来检测病人血清ＴＳＡｂ和甲状腺刺激阻断抗体（ＴＳＢＡｂ），均具有较好的灵敏度和特异性。
The culture of human normal and hyperfunctioning thyroid tissue was studied to determine the growth conditions and reactivity of the thyroid cells and to evaluate its application in detecting thyroid stimulating antibodies (TSAb) and thyroid stimulation blocking antibodies (TSBAb). The results showed that: (1)A large number of vital thyroid cells could be obtained from both thyroid tissues after digestion with trypsin and collagenase for 60～90 minutes; (2)cAMP released in response to both TSH and TSAb stimulation increased with cell density (5～20 ×104/well) and length of culture (0～96h) and (3) A high sensitivity and specificity were found with normal or hyperfunction thyroid cells to assay TSAb and TSBAb.
出处
《标记免疫分析与临床》
CAS
1994年第4期213-216,共4页
Labeled Immunoassays and Clinical Medicine
基金
国家自然科学基金
关键词
细胞培养
甲状腺
甲状腺机能亢进
TSH
TSAb
Cell. cultured/thyroid
Thyrotropin
Autoantibody/thyroid
Adenosine cyclic monophosphate
