摘要
本文介绍前列腺特异抗原的纯化，免疫动物得到特异性抗ＰＳＡ抗体，并建成放射免疫分析。方法简便快速，灵敏度较高，准确性及重复性良好。与国外相应试剂盒比较有良好的相关性。
Human prostate specific antigen (hPSA) RIA was developed with hPSA and anti-PSA prepared in our laboratory. Its standard curve was linear with a sensitivity of 0.5μg/L. Serum PSA levels of 130 normal males ranged from 0 to 3.5μg/L (1.15±0.93μg/L),which are equiralent to the results of other conventional RIA. The recovery,intra-and inter-assay coefficients of variation conformed to the demands of RIA,and the results of 41 samples obtained by both the PSA RIA and PSA of DPC were well correlated (r=0.990).PSA level of 23 patients with prostatic carcinoma was 10～400μg/L.
出处
《标记免疫分析与临床》
CAS
1994年第3期160-163,共4页
Labeled Immunoassays and Clinical Medicine
关键词
前列腺特异抗原
放射免疫分析
前列腺肿瘤
Prostate specific antigen
Radioimmunoassay
Prostate neoplasms/diagnosis
