摘要
目的探讨2,3,7,8四氯苯二英(TCDD)对NIH小鼠胚胎发育过程的影响。方法在NIH小鼠妊娠早期的1～8d,胚泡着床前期的1～3d和着床后期的4～8d,经口腔灌服0、2、50和100ng(kg·d)剂量的TCDD,在小鼠妊娠第9天和第18天时观察子宫内的胚胎发育形态。结果50、100ng(kg·d)剂量在妊娠早期1～8d染毒,使多数妊娠小鼠在第9天观察时子宫内胚胎全部丢失;在妊娠1～3d和4～8d染毒,使部分妊娠小鼠胚胎丢失,另外有部分胚胎吸收或发育阻滞。同时发现早期成活胚胎在发育过程中继续死亡,妊娠第18天时,胎鼠出生前成活率下降。结论小鼠妊娠早期染毒,引起小鼠妊娠早期胚胎丢失,着床后胚胎发育异常,出现阻滞和死亡。
Objective To determine the effects of tetrachlorodibenzo p dioxin (TCDD) on embryo development of NIH mice. Methods Groups of pregnant mice, 10 mice in each group, were orally administrated with TCDD in a dose of 0, 2, 50 or 100 ng·(kg·d) -1 during 1～8 d, 1～3 d, and 4～8 d of pregnancy. The animals were sacrified and embryo developmental data were collected at day 9 or day 18 Results\ Most animals lost all their embryos when checked at day 9 when the pregnant mice were dosed with 50 and 100ng·(kg·d) -1 TCDD during 1～8 d of pregnancy. A part of animals lost embryos, and others absorbed or retarded at stages when dosed with 50 or 100ng·(kg·d) -1 TCDD during 1～3 d and 4～8 d. The toxic effects of TCDD maintained during the whole development period. The survived embryos continue to lose after dosing, leading to a further declined survival rate at day 18 Conclusion Administration of TCDD during early pregnancy can cause loss of early embryos and developmental abnormalities of implanted embryos, and leading to further development retardation even death of late embryos.
出处
《中国比较医学杂志》
CAS
2005年第3期150-153,共4页
Chinese Journal of Comparative Medicine
基金
国家自然科学基金(30371196)项目资助
