摘要
的研究基质金属蛋白酶-1(MMP-1)在非小细胞肺癌组织中的表达及临床意义。方法应用免疫组织化学方法和Westernblotting检测70例非小细胞肺癌组织中MMP-1蛋白的表达,并进行图像分析测定电泳条带的吸光度A值。结果70例非小细胞肺癌和30例正常肺组织中MMP-1表达阳性的例数分别为33例(47.1%)和7例(23.3%),差别具有显著意义。Ⅲ期肺癌的平均吸光度A值明显高于Ⅰ、Ⅱ期。有淋巴结转移癌的平均吸光度A值明显高于无淋巴结转移的病例,差异具有显著性意义(P<0.05)。MMP-1在肺腺癌和鳞癌中的表达无显著性差异(P>0.05)。结论MMP-1在非小细胞肺癌组织中的表达增多,与非小细胞肺癌分期、淋巴结转移有关。
Objective To explore the expression level of MMP-1 in patients with primary non-small cell lung cancer (NSCLC). Methods The expression of MMP-1 protein was detected in 70 NSCLC tissues by immunochemistry and Western blotting followed by image analysis in order to detect the A value of expression. Results The positive rate of MMP-1 expression in NSCLC tissues was 47.1% (33/70), higher than that in normal lung tissue. There was no significant difference in MMP-1 expression between NSCLC tissues of squamous cell carcinoma and adenocarcinoma(P>0.05). The A value of MMP-1 expression in NSCLC tissues of Ⅲ stage was significantly higher than that in NSCLC tissues ofⅠ,Ⅱstages ( P<0.05). The A value of MMP-1 expression in NSCLC tissues of N1,2,3 groups was significantly higher than that in NSCLC tissues of N0 group ( P<0.05). MMP-1 expression was related to lymph node metastasis (P<0.05) and TNM stages of lung cancer (P<0.05), but not to the histological classification. Conclusion MMP-1 protein expression may be associated with the invasion and progression of NSCLC.
出处
《现代肿瘤医学》
CAS
2005年第3期329-331,共3页
Journal of Modern Oncology
