摘要
目的:研究慢性束缚应激时大鼠海马脑啡肽和前强啡肽mRNA基因表达的变化以及逍遥散、四君子汤、金匮肾气丸三种中药复方对其的影响。方法:用特制束缚架连续束缚7d与21d,每天3h的方法制作大鼠束缚应激模型;以RT PCR反应,扩增脑啡肽和前强啡肽基因,同时以βactin作为内对照,用凝胶图像分析系统进行扫描并分析,把目的基因的光密度与内参照条带的光密度进行比较后进行半定量分析。结果:7d模型组大鼠海马前强啡肽mRNA的表达明显增强(P<0.01),21d模型组海马脑啡呔mRNA和前强啡肽mRNA的表达明显增强(P<0.01);三个复方均能降低海马内前强啡肽mRNA的表达(P<0.01),逍遥散和四君子汤能降低脑啡呔mRNA的表达(P<0.01)。结论:逍遥散对脑啡呔mRNA前强啡肽mRNA的基因表达的改善作用明显优于金匮肾气丸组。
Aim: To investigate the changes of enkaphalin mRNA and prodynorphin mRNA gene expression in rat brain regions with chro-(nic) immobilization stress and the influence of Chinese herbs. Methods: We copied the rat model of chronic immobilization stress(3 h daily , repeated 7 d or 21 d), and primers of enkaphalin or prodynorphin were respectively added for RT-PCR reaction(β-actin as inner contrast). Gel image analysis system was used to scan and analyze and odds of optical density of target gene and inner contrast strip were taken as quasi-quantified data. Results: Prodynorpin mRNA expression in hippocampus markedly increased in 7 d group (P<0.01), while enkaphalin mRNA prodynorphin mRNA expression in hippocampus markedly increased in 21 d group(P<0.01). All three recipes were able to decrease the gene expression of prodynorphin mRNA(P<0.01), and Xiaoyao powder as well as Sijunzi soup were able to decrease the gene expression of enkaphalin mRNA(P<0.01).Conclusion: The effects of Xiaoyao powder on the above two gene expression was better than Jinkuishenqi pill.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2005年第2期121-125,共5页
Chinese Journal of Applied Physiology
基金
国家自然科学基金委(30000216)
高等学校全国优秀博士学位论文作者专项资金(200059)
霍英东教育基金会(81037)资助课题
