摘要
探讨降钙素抗血清制备的简便方法,用IPTG诱导重组鲑鱼降钙素(sCT)基因工程菌BL2 1(pGEX 4T sCT) ,超声波裂解后,用亲和层析凝胶纯化蛋白GST sCT ,并以此融合蛋白为抗原制备抗血清。实验证明:降钙素是1种半抗原;大肠杆菌表达的融合蛋白GST sCT只具有鲑鱼降钙素免疫原性,而无谷胱甘肽免疫原性。经鉴定,制备的免疫血清具有良好的特异性和敏感性。
After being induced with isopropylthio-β-D-galactoside(IPTG), fusion protein, glutathione-S-transferase-salmon calcitonin(GST-sCT), was purified using Glutathione Sepharose 4B affinity chromatography from the bacterium BL21(DE3) containing recombinant plasmid pGEX-4T-sCT. The purified GST-sCT was used as antigen to immune rabbit in order to prepare the antiserum of salmon calcitonin. The GST-sCT has the immunogenicity of sCT only, verifying that calcitonin is a hapten, and it is identified that the prepared antiserum has high specificity and sensitivity for sCT.
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2005年第3期507-510,共4页
Periodical of Ocean University of China
基金
国家高技术研究发展计划项目 (81 9 0 4 1 0 )资助
关键词
鲑鱼降钙素抗血清
融合蛋白
免疫原性
salmon calcitonin antiserum
fusion protein
immunogenicity
