摘要
将猪繁殖与呼吸综合征病毒(PRRSV)的核衣壳蛋白(N蛋白)基因克隆到原核表达载体pET 28α(+)中,得到重组质粒pET PRRSV/N,并转化入受体菌BL21中。转化子经IPTG诱导表达4h后,表达蛋白量达到高峰。经15%SDS PAGE电泳检测和经Westernblotting分析,表达蛋白大小约为17kD,符合预期结果,并能与PRRSV阳性血清发生特异性反应,而不与阴性血清反应。这说明表达蛋白具有高度免疫反应特异性。
<Abstrcat>The nucleocapsid protein(N) gene of porcine reproductive and respiratory syndrome virus(PRRSV) was cloned into the multiple cloning site of pET-28α(+) vector.The recombinant of pET-PRRSV/N was cultivated and induced with IPTG.The amount of expressed protein reached a high peak 4 hours after the induction. The examination analysis results of the protein by using SDS-PAGE and Western blotting showed that the protein was 17 kD in size and specifically reacted with PRRS-positive sera from pigs,not negative sera,indicating the recombinant protein had high specificity of immune reaction and can be used as antigen of diagnostic assay for PRRS in practice.
出处
《浙江农业学报》
CSCD
2005年第3期123-126,共4页
Acta Agriculturae Zhejiangensis
基金
浙江省科技厅重点项目资助(011102102)
