摘要
通过光镜、扫描电镜和透射电镜的观察对海湾扇贝血细胞的形态、表面结构及超微结构进行了研究。根据细胞的大小、形态结构可将血细胞分成四种类型:Ⅰ型小透明细胞,大小约(2·38±0·08)μm,约占比例30%-35%;Ⅰ型大透明细胞,大小约(4·41±0·33)μm,约占比例15%-25%;Ⅱ型小颗粒细胞,大小约(4·15±0·26)μm,约占比例20%-25%;Ⅱ型大颗粒细胞,大小约(8·26±0·52)μm,约占比例25%-30%。血细胞在血淋巴中的平均密度为(3·75±0·65)×107cell/ml。其中Ⅰ型透明细胞占55·3%,Ⅱ型颗粒细胞占44·7%。表面结构观察结果显示有5种形态:圆形血细胞,梨形或梭形血细胞,松果形血细胞,阿米巴样细胞,大型细胞,表面结构与功能密切相关。透射电镜观察结果表明血细胞主要归属于两大类型:Ⅰ型透明细胞和Ⅱ型颗粒细胞。超微结构显示颗粒细胞的细胞质颗粒可区分成三种类型:Ⅰ型高电子密度颗粒,Ⅱ型低电子密度颗粒和Ⅲ型中等电子密度颗粒,并推测Ⅰ型高电子密度颗粒是细胞吞噬的异物(微生物等)或细胞内的废物(沉积颗粒,衰老的胞器或碎片);Ⅱ型低电子密度颗粒是溶酶体类的胞内分泌颗粒,来源于高尔基复合体或内质网;Ⅲ型中等电子密度颗粒可能是次级溶酶体,由Ⅰ型颗粒向Ⅱ型颗粒融合并注入裂解酶类而形成[动物学报51(3):486-494,2005]。
Light, transmission electron and scanning electron microscopical studies were carried out to characterize the haemocytes in the scallop Argopecten irradians. Fore kinds of haemocytes were recognized: typeⅠsmall hyalinocytes, size of (2.38±0.08) μm, percentage of 30%-35%; typeⅠlarge hyalinocytes, size of (4.41±0.33) μm, percentage of 15%-20%; typeⅡsmall granulocytes, size of (4.15±0.26) μm, percentage of 20%-25%; typeⅡ large granulocytes, size of (8.26±0.52) μm, percentage of 25%-30% according to cell size, granular features, and some structural and functional characteristics. There might be 5 types of surface structures in the haemocytes under scanning electron microscopy:round shape cells, pear or spindle shaped cells, pinecone-like cell, amoeba-like cells and large shaped cells. Granulocyte types showed larger sizes and smaller N/C ratios than hyalinocytes. The mean haemocyte number was about(3.75 ± 0.65)×10 7 cells/ml of haemolymph. Differential haemocyte counts identified the haemocyte population containing 44.7% granular cells and 55.3% agranular cells. Three types of granules were distinguished: typeⅠ, with high electron-dense, size of 0.1-0.6 μm, with or without membrane surrounding it; typeⅡ,with low electron-dense, size of 0.1-0.5 μm, usually surrounded by a membrane; type Ⅲ, with medium electron-density, size of 0.2-1.0 μm, usually surrounding by a membrane. We suppose that the typeⅠgranules might be formed by engulfing the foreign materials (microorganisms, or other granules) or internal waste materials (organelles or fragments); the typeⅡ granules might be formed by primary lysosomes, probably originating in the Golgi complex or endoplasmic reticulum; and the type Ⅲ granules might be the secondary lysosomes and formed by fusion of two kinds of typeⅠand typeⅡgranules or the matured typeⅠgranules which absorbing some hydrolases in it.
出处
《动物学报》
SCIE
CAS
CSCD
北大核心
2005年第3期486-494,共9页
ACTA ZOOLOGICA SINICA
基金
国家自然科学基金农业倾斜项目(No.30170741)
面上项目(No.30371107)资助~~
