摘要
目的观察肾小球肾炎患者肾组织中乙型肝炎病毒(HBV)表达情况,探讨肾组织HBV抗原的来源及其与组织病变的关系。方法应用于免疫组化(S-P)法检测肾炎肾组织中的HBV抗原(HBsAg、HBcAg)。5例膜性肾病作透射电镜观察。结果血清HBV感染标志阴性肾炎与阳性者的肾组织中HBV抗原沉积一致,且肾小管HBcAg高表达。肾小管HBcAg阳性组的肾组织病变程度明显严重于阴性组,差异有显著性(P<0.05)。电镜下,血清HBV标志阴性肾炎与阳性者表现不同。结论肾组织中HBV抗原抗体免疫复合物有原位形成的可能;血清HBV感染与肾炎〔膜性肾病(MGN)、膜增生性肾小球肾炎(MPGN)、系膜增生性肾小球肾炎(MsPGN)、IgA肾病(IgAN)〕肾组织感染HBV不一致。肾小管中HBcAg可能与上述肾炎的肾组织病变程度有关。
Objective To study the expression and origin of HBV in renal tissue of glomerulonephritis, and the relationship between HBV infection and pathology change of glomerulonephritis. Methods HBV antigens (HBsAg, HBcAg) in renal biopsy specimens with various types of glomerulonephritis were examined by immunohistochemical techniques(S-P). By electron microscopy examination there were 5 patients with membranous nephropathy. Results The deposits of HBV marker were coincident in the renal tissue of glomerulonephritis with negative serum HBV and HBV antigenemia. The positive rate of HBcAg in the tubular cells was higher.Pathological change degree in the renal tissue was statistically significantly more serious in HBcAg positive group in tubular cells than in negative group(P<0.05). Electron microscopy demonstrated the changes in renal tissue were different between negative serum HBV and HBV antigenemia. Conclusion The results suggest that it is possible that the deposit of HBV antigen antibody immunocomplex in renal tissue originated from renal cells in situ, and the infection of HBV in serum and renal tissue of certain cases of glomerulonephritis (MGN、MPGN、MsPGN、IgAN ) is incongrous. The deposit of HBcAg in the renal tubular may be related with the degree of pathological changes of above cases of glomerulonephritis.
出处
《山西医科大学学报》
CAS
2005年第3期295-298,共4页
Journal of Shanxi Medical University
关键词
肾小球肾炎
肝炎病毒
乙型
抗原
抗体
glomerulonephritis
hepatitis B virus
antigens
antibodies
