摘要
应用大鼠肠系膜上动脉夹闭模型,对肿瘤坏死因子(TNF)在肠缺血所致多脏器损伤中的作用进行了探讨。结果发现,肠缺血再灌注初期循环 TNF 水平即显著升高,其动态变化与门脉系统内毒素血症密切相关;预防性给予 TNF-McAb 治疗则可有效地减轻机体的全身性损害。提示:严重损伤早期体内 TNF 的过度产生、释放,对宿主肝、肾及肺等器官功能有明显影响,这可能是导致脓毒症、多系统器官功能衰竭等并发症的重要原因之一。
To explore the role of tumor necrosis factor (TNF) in the pathogenesis of multiple organ dysfunction following bowel ischemia and reperfusion,98 rats were subjected to occlusion of the superior mesenteric artery for 45 minutes.It was found that the plasma TNF level increased rapidly after release of the clamp,peaking to 27.59± 11.13 ng/ml 2 hours after reperfusion.Its changes in quantity was directly related to en- dotoxin in the portal circulation.Furthermore,the results showed that pretrearment with monoclonal antibody to TNF-α could significantly lowered the plasma TNF content and notably improved the functions of various organs.This study demonstrated that release of TNF might result in systemic hypotension and remarkable damage to liver;kidneys and lungs,which contributed to the development of sepsis and multiple system organ failure following severe ischemia-reperfusion injury of the intestine.
基金
全军"八五"协作攻关资助课题
关键词
肠缺血
肿瘤坏死因子
多脏器衰竭
Gut ischmia-reperfusion
Tumor necrosis factor
Endotoxemia
Multiple system organ failure
