摘要
应用聚合酶链反应（ＰＣＲ）产物直接测序，在国内首次发现１例持续高滴度乙型肝炎表面抗原（ＨＢｓＡｇ）和抗一ＨＢｓ共存者携带的乙型肝炎病毒ＤＮＡ第５３２位碱基Ａ被Ｇ取代，推导其“ａ”决定簇中第１２６位苏氨酸被丙氨酸取代，提示该株“ａ”决定簇第一个结构环疏水性增加，由此可能导致其抗原性改变，而诱发免疫逃避株形成。
e amplificated a HBV DNA
fraginent codingthe major HBsAg from a chronic HBsAg carrier's se-rum with high titer of HBsAg
and anti-HBs usingpolymerase chain reaction (PCR). The direct se-quencing of PCR products
revealed a single mutationfrom adnosine to guanosine at the nucleotide position532 of HBV
DNA of the escape mutant. This muta-tion might result in an aminoacid substitution
fromthreonine to alanime at the aminoacid position 126 ofHBsAg. Sinee this aminoacid is
located in the firstloop of the 'a' determinant of HBsAg, the mutationmight result in an alteration
of the stucturalantigenicity of the 'a' determinant and induce escapemutant of HBV.
出处
《中华医学杂志》
CAS
CSCD
北大核心
1994年第6期355-357,共3页
National Medical Journal of China
关键词
乙型肝炎病毒
基因结构
Hepatitis B virus Genes structuralPolymerase chain reaction DNA mutational ana-lysis
