摘要
２７例尿标本同时采用超速离心法，酚氯仿法抽提法、及原尿直接法，然后分别用于人类巨细胞病毒的聚合酶链反应检测。其阳性检出率分别为８５．１９％，５１．８５％和４８．１５％。经配比资料统计学分析表明，超速离心处理的阳性检出率显善高于抽提法和直接法。实验表明，原尿直接法的假阴性十分明显；而酚氯仿抽提处理亦无实际应用价值。唯有超速离心处理法可显著地提高尿巨细胞病毒的检测敏感性。
ltra-speed centrifugation(USC)of short time and phenol chloroform extraction(PCE) were em-ployed in preparing 27 urine samples and untreat urine sample(crude urine,CU),were used as templatesfor HCMV detection by PCR technique.The preliminary results showed that positive detection rateswere 85. 19%,51.85% and 48.15%,respectively.The data, after paired design chi square test demon-strated that the positive detection rate of USC was significantly higher that the other two methods. Weconcluded that USC treatment for urine sample was the best for HCMV detection using PCR technique.
关键词
巨细胞病毒
聚合酶链反应
尿液
Cytomegaloviruses Enzyme-linked immunosorbent assay
