摘要
成人Ｔ细胞白血病（ＡＴＬ）由人类Ⅰ型嗜Ｔ细胞病毒（ＨＴＬＶ－Ⅰ）引起，但其发病机理仍不清楚。我们应用逆转录－多聚酶链反应结合直接序列分析检测１０例ＡＴＬ患者的Ｎ－ｒａｓ基因，结果在一例ＡＴＬ发现Ｎ－ｒａｓ６１位点突变（ＣＡＡ→ＣＴＡ），说明ＨＴＬＶ－Ⅰ感染之后有多种因素参与ＡＴＬ的发病。
he Second Department of Internal medicine, KumamotoUniversity Medical School, JapanThe human T-lymphotropic retrovirus type Ⅰ (HTLV-Ⅰ) has been confirrned to be etiologically associated withadult T-cell leukernia (ATL), but the mechanism ofleukemogenesis in this disease is not clear. Somaticmutation of N-ras is now considered to be a critical stepin human leukemogenesis. We tested N-ras gene pointmutation in 10 patients with ATL using reversetranscription-polymerase chain reaction (RT-PCR) toamplify N-ras cDNA containing codons 12 , 13 and 61 ,followed by direct sequencing. In one case, mutationwas found at codon 61 that resulted in activation of thisgene. The leukemic cells from the remaining ninepatients did not show N-ras point mutation. Theseresults suggested that multifactors rather thanmonofactor involved in leukemogenesis of ATL afterHTLV-Ⅰ infection.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
1994年第7期356-358,共3页
Chinese Journal of Hematology
关键词
白血病
聚合酶链反应
基因突变
Adult T-cell leukemia Ras genePolymerase chain reaction DNAsequencing
