摘要
外周血ＣＤ＿（３４）抗原阳性，ＣＤ＿（３３）抗原阴性的细胞，在造血细胞生长因子（ＨＧＦ）──Ｇ－ＣＳＦ，ＩＬ－３和干细胞因子（ＳＣＦ）的联合作用下，可以在体外维持造血达９周，克隆形成细胞（ＣＦＣ）大量增加。ＣＦＣ和有核细胞增殖高峰在液体培养的第６～７周。在液体培养中不加入ＨＧＦ和单独应用Ｇ－ＣＳＦ的情况下，这类细胞不能在体外增殖。结果表明，ＨＧＦ的联合应用在维持外周血造血细胞的体外长期生长起主要作用。
nects of hema topoiet ic growth factors onperipheral blood cells were studied in a long term liquidculture system.The combination of G-CSF,IL-3 andstem ceil factor(SCF)was capable of sustaining thesecells to proliferate for up to 9 weeks and the number ofcolony forming cells(CFC ) was increased.The peak ofCFC and cell proliferation was about 6～7weeks.G-CSF alone showed no such effects.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
1994年第6期286-288,共3页
Chinese Journal of Hematology
关键词
造血细胞
生长因子
造血干细胞
Hernatopoietic growth factor Peri-pheral hematopoietic stem cell Long-term liquid culture
