摘要
用流式细胞仪（ＦＡＣＳｔａｒ）双抗体标记免疫荧光法，测定正常骨髓、化疗后的外周血和正常脐带血中的细胞及其亚群，发现总的细胞率在骨髓中（２．８９±０．９１％）和化疗后的外周血中（２．５８±０．７５％）较高，脐带血中较低（０．８８±０．４１％）。／ＬＩＮ￣＋细胞，包括等，在化疗后的外周血和脐带血中高于正常骨髓。ＬＩＮ￣－细胞，其中在骨髓中含量最高。ＣＦＵ－ＧＭ数与细胞绝对数的比例在化疗后的外周血中很低（１：２２），骨髓和脐带血两者的比例分别为１：１２和１：１３。提示化疗后外周血中的细胞的分化能力低于骨髓和脐带血。
s to quantitate the number of hematopoietic progeni-tors for an effective transplant,the hematopoieticstem cells and their subpopulations coexpressed by lin-eage-associated antigens from bone marrow (BM);um-bilical-cord blood(UB) and product of leukaphereses(LK)were evaluated by duol-color immunofluorescencewith FACStar.Total cells were higher in BM(2.89±0.91%)and in LK (2.58± 0.75%)than in UB(0.88±0.41%)./LIN￣+ cells,contained were elevated in LKand in UB.But/LIN￣-cells,especially cells,were much more in BM.The ratio between CFU-GM and cells,which represent the ability of differ-entiation of cells into myeloid lineage,was smallerin LK than in BM and in UB.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
1994年第5期230-232,共3页
Chinese Journal of Hematology
关键词
白血病
药物疗法
骨髓
CD34
T细胞
cells Bone marrow Periph-eral blood after chemotherapy Um-bilical cord blood
