摘要
采用甲基纤维素半固体体外培养法对脐带血造血祖细胞进行研究。人脐带血粒单祖细胞集落（ＣＦＵ－ＧＭ），红系爆式集落（ＢＦＵ－Ｅ）含量显著高于成人外周血，也高于骨髓（ＣＦＵ－ＧＭ，Ｐ＜０．０５；ＢＦＵ－Ｅ，Ｐ＜０．０１）。脐血造血祖细胞的出现晚于骨髓，但存活时间较长。在不外加促红细胞生成素时，脐血中有少量ＢＦＵ－Ｅ生成。将两份不同胎儿的脐血细胞混合后培养并与单个胎儿脐血细胞培养相比较，两者在祖细胞生成数量和生长特征方面无明显差别。采用单克隆抗体技术检测脐血中ＯＫＴ＿３，ＯＫＴ＿４，ＯＫＴ＿８阳性率，与成人相比，ＯＫＴ＿３￣＋降低，而ＯＫＴ＿４￣＋，ＯＫＴ＿８￣＋和ＯＫＴ＿４￣＋／ＯＫＴ＿８￣＋与成人相似。
n methylcellulose culture system, human cord bloodCFUGM , BFU-E were significantly higher than thoseof adult blood (P<0. 001) , and also higher than normalbone marrow (CFU-GM P<0. 05 ; BFU-E P<0. 01 ).The progenitor colonies appeared later in cord blood thanin adult bone marrow, but survived longer. Further-more , without adding Epo in culture system , there werea few BFU-E appeared in cord blood. Mixed culture oftwo human cord blood were evaluated. The similar re-sults with single cord cell culture suggested that the reac-tion between different cord lymphocytes did not affectthe growth of progenitors in cord blood.With the technique of monoclonal antibody, we foundthe mean proportion of OKT_3￣+ cells was lower in cordblood compared with those of adult blood , while the pro-portion of OKT_4￣+ /OKT_8￣+ ratio was similar to adult.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
1994年第4期178-180,共3页
Chinese Journal of Hematology
关键词
脐带血
造血祖细胞
淋巴细胞亚群
Cord blood hematopoietic progenitorT-lymphocyte subset
