摘要
应用吖啶橙（ＡＯ）检测细胞核酸含量，可同时获得ＤＮＡ与ＲＮＡ两项参数值．并根据两个参数确定细胞周期Ｇ０，Ｇ１，Ｓ，Ｇ２Ｍ的百分比值．ＡＯ与碘化丙啶（ＰＩ）两种方法测定药物对细胞周期的影响其结果基本相同．不同的是ＡＯ可以单独观察到Ｇ０期的变化，系用一个激发光源可获得两项参数的唯一染料．
By using acridine orange (AO) for nucleic acid assay by flow cytometry, two parameters of celluar DNA and RNA were determined simultaneously. And in the light of the value of these two parameters,G0 phase could be identified and distinguished from G1, so that phase of G0,G1,S,G2M in cell cycle were examined in percentage.The results by AO assay were consistent with that by PI. AO is an useful and unique stain to observe G0 phase and to obtain two parameters of DNA/RNA with one excited light source in FCM.
出处
《中华物理医学杂志》
CAS
CSCD
1994年第3期166-169,共4页
Chinese Journal of Physical Medicine and Rehabilitation
关键词
吖啶橙
流式细胞术
双参数
acridine orange
flow-cytometry
dual-parameter
