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毛细管电泳检测神经细胞凋亡 被引量：2

Measurements of PC12Cells Apoptosis with Capillary Electrophoresis
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摘要 建立了毛细管电泳检测凋亡细胞DNA片段的方法。以DNA相对分子质量标准品为溶质,考察了分离条件(电压、进样时间、温度、聚合物浓度)对分离的影响。在优化条件下,利用毛细管无胶筛分电泳对缺氧缺血过程中不同时间点神经PC12细胞DNA片段进行了分析,并与流式细胞仪结果比较,研究缺氧缺血时胶质细胞凋亡过程。 A capillary electrophoresis method was developed to characterize the DNA damage of apoptosis.The effects of experimental parameters(separation voltage,injection time,temperature and polymer concentraˉtion)on the separation efficiency were studied using100bp DNA ladder as the analyte.Under optimized conˉditions,the DNA of PC12treated with ischemia and anoxia was analyzed by non_gel sieving capillary elecˉtrophoresis.The dynamic apoptosis process of PC12cells was also studied by the above method.
作者 谢敏杰 朱舟 余勇飞 王伟 徐运兰
机构地区 华中科技大学同济医学院附属同济医院神经内科研究室
出处 《分析测试学报》 CAS CSCD 北大核心 2005年第3期37-40,共4页 Journal of Instrumental Analysis
基金 国家自然科学基金资助项目(30230140 30400142) 武汉市青年晨光计划资助项目(20035002016-19)
关键词 毛细管电泳 星形胶质细胞 凋亡 DNA Capillary electrophoresis PC12cell Apoptosis DNA
分类号 O657.8 [理学—分析化学] Q523 [生物学—生物化学]
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同被引文献18

  • 1沈佐君,白洁,丛玉隆.电泳技术在临床检验中的应用[J].中华检验医学杂志,2005,28(7):684-686. 被引量：8
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分析测试学报
2005年 第3期

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