摘要
以正交设计法，采用了正交表Ｌ８（４１×２４）研究了念珠菌对上皮细胞体外吸附的诸条件，包括４因素及２～４水平，分别比较各因素在不同水平下的吸附率。结果提示，促进吸附的最佳组合因素是：念珠菌菌相（Ｅ＝３４．２６），孵育１２小时（Ｄ＝３３．７０），念珠菌与细胞间比率为１：２００（Ｃ＝３４．８８）以及孵育温度在３７℃时（Ｂ＝３３．８６）。以４种物质孵育后均可抑制菌对细胞的抑制率，其中尤以甘草甜素的作用为最强。由此也提示了器官表面（如口腔，肠道）常驻的真菌的数量对念珠菌病的发病极为重要，如能服用一些中药，如甘草甜素将是可能控制发生念珠菌病的一种措施。
In order to explore the in vitro conditions about the adherence of Candida to epithelium, an orthogonal design L8 (41 24) including 4 factors and 2--4 levels to compare the percentage of adherences in different levels of each factor was tested. The results showeds that the optimum combination factors enhancing adherence are as follows: phase of Candida (E=34.26); incubation period 12 h (D=33.70); ratio of Candida to cultured cells l:200 (C=34.88); incubation temperature 37 (B=33.86). All four material. tested could inhibit the adherence of C. albicans, among which glycirrhizine is the most poteds. It also indicated that the hequantity of fungi colonized on the surfaces of organs (mouth, intestine) is important to the pathogenesis of candidiasis, and the administration of some Chinese herbal medicines such as glycyrrizine probably could protect hosts from candidiasis.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
1994年第1期22-25,共4页
Chinese Journal of Dermatology
基金
自然科学基金资助课题
