摘要
应用合成肽酶联免疫吸附试验（ＥＬＩＳＡ）和重组免疫印迹试验（ＲＩＢＡ），动态观察了１０例输血后肝炎（ＰＴＨ）患者丙型肝炎病毒（ＨＣＶ）感染早期（输血后３６～３８周）的抗体应答规律，发现９例血清抗ＨＣＶ阳性，其中８例ＨＣＶ－ＲＮＡ阳性。核心（Ｃ）抗体检出率高，其出现时间比非结构（ＮＳ）区抗体提前１～３周。Ｃ区合成肽抗体；ｌｇＭ比ｌｇＧ提前１～４周检出，其检出的吸光值与血清ＡＬＴ（ＧＰＴ）水平呈显著正相关（Ｐ＜０．０１）。同时发现３例存在抗ＨＣＶ“被动输入”。研究表明，本地区ＰＴＨ患者多由ＨＣＶ感染所致。检测Ｃ区抗体ＩｇＭ不仅有助于早期诊断，而且可反映ＨＣＶ活动性感染。
echniques of enzyme-linked immunosorbent assay based on synthetic multiple peptide fragments and second generation recombinant immunoblot assay(RIBA)were used to study the patterns of specif-ic antibody response in 10 cases of posttransfusion hepatitis(PTH)during a period of 36～38 weeks af-ter blood transfusion. Nine cases were positive with serum anti-HCV,including 8 cases positive with se-rum HCV-RNA. Antibodies to core protein of HCV showed a higher positive rate and were detected 1～3 weeks earlier than those to the putative nonstructural(NS) protein. Anti-HCV lgM to core pro-tein were detected 1～4weeks earlier than anti-HCV lgG and the detective absorbent values of anti-HCV IgM were positively correlated with the levels of serum ALT(P<001).“passive transfer” of anti-HCV were found in 3 cases.These facts suggest that HCV is the major causative agent of PTH cases in our district and anti-HCV IgM to core protein is a putative serological marker not only for ear-ly diagnosis of HCV infection but also for demonstration of active HCV infection.
出处
《中华内科杂志》
CAS
CSCD
北大核心
1994年第9期593-596,共4页
Chinese Journal of Internal Medicine
