摘要
目的探讨持续吸入高氧后新生大鼠肺组织一氧化氮(NO)含量、一氧化氮合酶(NOS)活力的动态变化及病理学改变。方法足月新生鼠生后12h内分别持续吸入90%±5%的高氧和空气,于1、3、7、14、21d,分别检测肺组织NO含量、NOS活性以及动态观察病理改变。结果(1)NO含量:在7、14和21d,高氧组水平高于空气组,数值分别为(99.38±7.80)vs(88.78±8.00),P<0.05;(128.18±33.78)vs(93.30±16.73),P<0.05;(170.66±34.00)vs(106.37±25.11),P<0.01。(2)NOS活力:在高氧暴露3d时高于空气组,(20.56±2.56)vs(18.25±0.71),P<0.05;并持续至21d,数值分别为(24.09±2.48)vs(21.10±2.38),P<0.05;(25.07±2.06)vs(20.27±4.15),P<0.05;(27.06±4.79)vs(20.45±2.53),P<0.01。(3)病理学:吸高氧3d时炎症反应为主,7d时炎症反应更明显,开始出现肺间隔增宽,肺泡发育受阻,14d和21d间质增生、肺泡化降低越来越明显。结论持续吸入高氧可致新生鼠发生与人类BPD有类似的病理改变;肺组织病理损伤逐渐加重时,肺组织NO含量和NOS活性增加,提示NO可能在BPD中有重要作用。
Objective To determine dynamic changes of contents of nitric oxide(NO) ,nitric oxidesynthase(NOS) activity in lung tissues and pathological changes in lung of neonatal rats after inhaling high concentration of oxygen.Methods Full-term newborn rats were continuously exposed to oxygen (90%±5%) or room air (21% O_2) after birth within 12 hours.Dynamic changes of content of NO,NOS activity in lung tissue and lung pathological changes were monitered on days 1,3,7,14 and 21 in hyperoxia groups and controls.Results (1)contents of NO:on 7,14,21d were higher than control,datas were:(99.38±7.80) vs (88.78±8.00),P<0.05;(128.18±33.78) vs (93.30±16.73),P<0.05;(170.66±34.00) vs (106.37±25.11),P<0.01.(2) NOS activity:on 3d was higher than control,data was:(20.56±2.56) vs (18.25±0.71),P<0.05;lasted to 21d,datas were:(24.09±2.48) vs (21.10±2.38),P<0.05;(25.07±2.06) vs (20.27±4.15),P<0.05;(27.06±4.79) vs (20.45±2.53),P<0.01.(3) lung pathological changes:in hyperoxia group,inflammatory response was seen on 3d of inhaling high concentration of oxygen,on 7d inflammation was more evident,alveolar septa began to widen with arrested development of alveoli,on 14d and 21d interstitial hyperplasia and decreased alveolarization were more and more evident.Conclusion After prolonged inhaling high concentration of oxygen,neonatal rats show a lesion that is very similar to human BPD ,and contents of NO,NOS activity in lung tissue increased when lung injury was aggravated,which implicate a possible role of NO in the development of BPD.
出处
《小儿急救医学》
2005年第3期177-179,共3页
Pediatric Emergency Medicine
基金
辽宁省教育厅高等学校科学研究项目(基金号202013127)
