摘要
目的探讨系统性红斑狼疮(SLE)患者外周血淋巴细胞糖皮质激素受体及T淋巴细胞亚群的变化。方法用放射性配基结合一点分析法及碱性磷酸酶抗碱性磷酸酶法,测定30例SLE患者外周血淋巴细胞糖皮质激素受体及T淋巴细胞亚群,并与20例正常对照组相比较。结果SLE患者外周血淋巴细胞糖皮质激素受体为3654±459结合位点细胞,与正常对照组(4839±575结合位点细胞)相比,显著降低,差异有显著性(P<0.01);SLE患者外周血CD3+T细胞、CD4+T细胞及CD4+CD8+比值与正常对照组相比,显著降低,差异有显著性(P<0.01);CD8+T细胞亦降低,与正常对照组相比,差异无显著性(P<0.05)。结论SLE患者存在免疫调节功能的紊乱,SLE患者外周血淋巴细胞的糖皮质激素受体水平降低,可能在一定程度上导致了免疫调节紊乱,从而参与了SLE的发病。
Objective: To investigate the relationship between glucocorticoid receptors (GR) and the T-cell subset in systemic lupus erythematosus (SLE). Methods: GR and the T-cell subset were measured by GR radioligand-binding assay and alkalinephosphatase anti-alkalinephosphatase (APAAP) assay respectively in peripheral blood lymphocytes from 30 cases of SLE and 20 normal controls. Results: The GR number (3 654±459) binding sites/per cell in peripheral blood lymphocytes in SLE were decreased significantly, compared to the normal controls (4 839±575, P<0.01). The CD3+ and CD4+ cell subtypes, and CD4+/CD8+ ratio in peripheral blood lymphocytes in SLE patients were significantly decreased compared to normal controls (P<0.01). The CD8+ cell subtypes were also decreased, but they did not significantly differ from the normal controls (P>0.05). ~Conclusion : The changes of GR number per cell in peripheral blood lymphocytes of SLE may be involved in the immunoregulatory dysfunctions in pathogenesis of SLE.
出处
《中国麻风皮肤病杂志》
2005年第6期419-421,共3页
China Journal of Leprosy and Skin Diseases
