摘要
目的:研究益气活血软坚解毒(YHRJ)方含药血清诱导人肝癌细胞系BEL7402细胞内Ca2+、线粒体膜电位(△ψm)变化。方法:YHRJ方组成:生黄芪、炒白术、田三七、水红花子、藤梨根、凌霄花、炮山甲、白花舌蛇草,半枝莲。将新西兰大耳白兔12只随机分为3组,每组各4只,分别灌服生理盐水、等效剂量YHRJ方(约为人用量的5倍)与高剂量YHRJ方(约为人用量的10倍)水煎剂,用药5次后制备3种含药血清。BEL7402细胞分为生理盐水组、生理盐水+顺铂氨铂(NS+DDP)组、YHRJ等效剂量(YHRJD)组、YHRJ等效剂量+顺铂氨铂(YHRJD+DDP)组、YHRJ高剂量(YHRJG)组、YHRJ高剂量+顺铂氨铂(YHRJG+DDP)组,分别加入生理盐水、YHRJ等效剂量、YHRJ高剂量兔血清至终浓度100ml/L,DDP至终浓度4.5mg/L,孵育8、12、24、48、72h后分批处理细胞,罗丹明123、Fluo3染色,流式细胞术(FCM)检测细胞内Ca2+、线粒体膜电位水平。结果:与NS组相比,在12、24、48h各加中药组细胞内Ca2+浓度普遍增高(P<0.05)。在12h,各加中药组膜电位普遍增高(P<0.05),出现超极化现象;在24h,与前基础电位相比,各加中药组线粒体膜电位明显降低(P<0.05);在48h,各加中药组膜电位也保持在相对低的水平。结论:YHRJ含药血清有促进细胞内Ca2+内流与降低线粒体膜电位的作用。
Objective:To study the effects of Yiqi Huoxue Ruanjian Jiedu(YHRJ) drug serum on Ca 2+ and mitochondrial membrane potential (△ψm) in human hepatocellular carcinoma cell line BEL-7402.Methods: YHRJ decoction was composed of Astragalus Root, White Atractylodes Rhizome, Radix Notoginseng, Fructus Polygoni Orientalis, Radix Actindiae Argutae, Flos Campsis, Pangolin Scales, Oldenlandia diffusa Roxb, and Herba Scutellariae Barbatae. Twelve New Zealand rabbits were randomly assigned into 3 groups: NS group, YHRJ equivalent dose group(YHRJD) and YHRJ high dose group(YHRJG), which were fed with NS,YHRJ equivalent dose decoction (5 times as large as normal human dose) and high dose YHRJ decoction(10 times as large as normal human dosage), respectively. Drug serum was prepared after the rabbits were fed with drugs for 5 times. BEL-7402 cells were divided into 6 groups according to different drugs, including NS group, NS+DDP group, YHRJ equivalent dose group, YHRJ equivalent dose+DDP group, YHRJ high dose group, and YHRJ high dose +DDP group. The concentrations of NS,YHRJD and YHRJG were adjusted to 100 ml/L ,and the concentration of DDP was adjusted to 4.5 mg/L. Flow cytometry was applied to detect the concentrations of Ca 2+ and △ψm 8, 12, 24 and 48 h after the drugs were added.Results: The Ca 2+ concentrations at 12, 24 and 48 h after treatment were significantly higher than those of the control group(P<0.05). Compared with control group, the △ψm at 12 h after treatment in YHRJ equivalent dose group, YHRJ equivalent dose+DDP group, YHRJ high dose group, and YHRJ high dose+DDP group was significantly higher and superpolarization appeared(P<0.05). However, compared with baseline △ψm, there was a decrease in △ψm at 24 h in YHRJ equivalent dose group, YHRJ equivalent dose+DDP group, YHRJ high dose group, and YHRJ high dose+DDP group (P< 0.05), and △ψm remained at a low level at 48 h.Conclusion: YHRJ drug serum may induce apoptosis through inhibiting △ψm and promoting Ca 2+ inward flow.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2005年第6期667-670,共4页
Academic Journal of Second Military Medical University
关键词
中草药
肝肿瘤
CA^2+
线粒体膜电位
drugs,Chinese herbal
liver neoplasms
Ca 2+
mitochondrial membrane potential
