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IL-10启动子单核苷酸多态性与肾移植急性排斥:供受者配对研究

Interleukin-10 promoter single nucleotide polymorphism and acute rejection in renal transplantation: a donor-recipient paired study
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摘要 目的:以供受者配对的方式,研究IL10启动子区-1082、-819和-512位点单核苷酸多态性(SNP)对肾移植急性排斥的影响。方法:应用序列特异性引物聚合酶链反应(SSPPCR)方法,测定52对首次肾移植的供受者的IL-10启动子区-1082、-819和-512位点的SNP,确定其单倍体型及对应的表达型。随访观察移植后1年内急性排斥的发生情况。采用Fisher精确检验,比较不同配对方式之间急性排斥发生率的差异。结果:本组供、受者中均无对应高表达型的单倍体型。随访1年者49例,18例(36.7%)患者发生急性排斥反应,发生多发急性排斥(MR)7例(14.3%)。供者IL-10中表达型与急性排斥和MR间的关系有统计学趋势(P值分别为0.076和0.051),供者中表达型/受者低表达型的配对中MR发生率增加显著(P=0.036)。结论:研究IL-10SNP与肾移植急性排斥的关系时,应该考虑供者的SNP类型。供者中表达型与急性排斥和MR相关联,供者中表达/受者低表达的配对方式与MR相关联。 Objective:To study the influence of 3 single nucleotide polymorphisms (SNP) of IL-10 promoter (-1 082, -819 and -594) on acute rejection after renal transplantation in a donor-recipient paired mode. Methods: Three IL-10 promoter SNPs of 52 donor-recipient pairs were genotyped and phenotyped by PCR using sequence-specific primers (SSP). Recipients were followed up for 1 year after transplantation, and acute rejection episodes were observed. Incidences of rejection were compared against pair patterns and differences were analyzed using Fisher's exact test. Results: No genotype of high producer was found in any donor and recipient in this study. Forty-nine recipients were followed up for over 12 months. Of them, 18 recipients (36.7%) had acute rejection and 7 recipients (14.3%) had multiple rejection(MR). The relationship between donors with intermediate producer and acute rejection and MR tended to be statistically significant (P=0.076, P=0.051).The MR rate increased significantly in the pair of donor intermediate producer/recipient low producer (P=0.036). Conclusion: It is preferable to take donor’s IL-10 SNP into consideration in study of IL-10 SNP and renal transplantation. Donor intermediate producer can increase the risk of acute rejection. The combination of donor intermediate/recipient low producer can increase the risk of MR.
作者 梁军号 羊继平 齐隽
机构地区 第二军医大学长征医院泌尿外科 解放军第
出处 《第二军医大学学报》 CAS CSCD 北大核心 2005年第6期651-653,共3页 Academic Journal of Second Military Medical University
基金 全军医药卫生科研"十五"规划指令性课题(01Z061).
关键词 白细胞介素10 多态性 单核苷酸 肾移植 移植物排斥 interleukin-10 polymorphism,single nucleotide kidney transplantation graft rejection
分类号 R692 [医药卫生—泌尿科学]
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参考文献9

  • 1Sankaran D, Asderakis A, Ashraf S. Cytokine gene polymorphisms predict acute graft rejection following renal transplantation[J]. Kidney Int, 1999,56(1): 281-288.
  • 2Hutchings A, Guay-Woodford L, Thomas JM. Association of cytokine single nucleotide polymorphisms with B7 costimulatory molecules in kidney allograft recipients[J]. Pediatr Transplant, 2002, 6(1): 69-77.
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  • 4田野,马威然,张玉海.细胞因子基因型多态性与肾移植排斥反应的关系[J].中华外科杂志,2002,40(4):256-258. 被引量：8
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二级参考文献9

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  • 9Turner D,Grant S,Yonan N,et al.Cytokine gene polymorphism and heart Transplant Rejection[].Transplantation.1997

共引文献7

第二军医大学学报
2005年 第6期

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