摘要
目的探讨乙醇首过代谢发生的部位,从而为酒精性疾病的治疗提供新的思路。方法Wistar大鼠40只随机分成两组:模型组24只,对照组16只,建立大鼠酒精模型。用酶组织细胞化学染色技术,在光镜下观察肝胃乙醇脱氢酶(alcoholdehydrogenaseADH)活性变化,并用灰度图像分析仪进行半定量。结果长期摄入乙醇后肝ADH活性进行性增加,胃ADH活性进行性下降,两者对照差异显著(P<0.05)。结论结合文献得出乙醇的首过代谢发生在胃;诱导胃ADH的活性,可降低乙醇对机体的毒性作用。
Objective To confirm the localization of the first pass metabolism of alcohol. Methods Forty wistar rats were divided into two groups: model group (24) and control group (16),at random. Then alcoholic models of rats wer e established .The activity varieties of hepatic and gastric alcohol dehydrogenases(ADH) were observed by optical microscopy in hepatic and gastric tissues stain ed by enzyme histo cytochemistry assay.The activity varieties were determined by LUZEX-F analysis instrument with a semi-quantitative method. Results It was shown that the activities of hepatic ADH gradually increased,and gastric ADH gradually decreased after long alcohol administration.There were si gnificantly differences between model group and control group (P<0.05),resp ectively. Conclusion The location of the first pass metabolism of alcohol is stomach by combining thi s test with literatures. Poisonous roles of alcohol will be decreased by induce ing activity of gastric ADH.
出处
《胃肠病学和肝病学杂志》
CAS
2005年第3期275-276,共2页
Chinese Journal of Gastroenterology and Hepatology
