摘要
目的:证实哺乳类耳蜗大上皮嵴和小上皮嵴分别能转化为内、外毛细胞。方法:采用体外培养wistar大鼠内耳基底膜,进行了大、小上皮嵴的定位和鉴定。结果:基底膜培养中,小上皮嵴细胞都能被毛细胞的早期标志肌球蛋白(myosinVIIa)染色,表明毛细胞分化,同时,在培养基中发现一些球囊型结构的细胞,细胞增殖标志(BrdU)、上皮细胞标志(ZO1)免疫组化阳性,表明该球囊不仅具有增殖能力,而且具有上皮特性。结论:建立了新生大鼠耳蜗铺片培养技术,识别和定位毛细胞前体细胞(即小上皮嵴和大上皮嵴区域),证明了小上皮嵴细胞和大上皮嵴细胞都具有转变成为毛细胞的潜能。
Objective: To establish the possibility of translation from greater epithelial ridge (GER) and lesser epithelial ridge (LER) of the mammalia cochlea into inner and outer hair cells respectively. Methods: Basememt membrane (BM) of wistar rats were cultured in vitro and orientation and identification of GER and LER were taken. Results: LER were dyed by myosin VIIa which proved hair cells differentiation in BM culture. Some saccule-like cells were found in the culture medium and the results of BrdU and ZO1 were positive by immunohistochemistry which proved proliferative capabilities and epithelium characteristics of these cells. Conclusion: Culture technique of newborn rats cochlea is successfully built, hair cell progenitors (LER and GER) are identified and located, and the translation from LER and GER into hair cells is established.
出处
《天津医药》
CAS
北大核心
2005年第6期366-368,F003,共4页
Tianjin Medical Journal
基金
中国博士后科学基金资助课题(项目编号:中博基2002第17号)
