摘要
以栽培种中芝５号、中芝８号、宜阳白和犀牛角为母本，以野生种为父本对其杂种胚的败育时间、营救技术及植株再生进行了研究。结果显示：授粉后９—１３天为杂种胚营救的较佳时期。ＭＳ矿质＋Ｎｉｔｃｈｅｒ有机营养＋ＢＡ０．２ｍｇ＋ＧＡ３０．１ｍｇ＋ＣＨ６００ｍｇ＋ＰＶＰ０．２％＋蔗糖５％能促进杂种胚的体外发育。幼胚在ＭＳ中萌发后采用ＭＳ＋ＰＰ３３３０．１ｍｇ＋ＢＡ０．２ｍｇ的培养基可壮苗促芽，在ＭＳ＋ＮＡＡ０．５ｍｇ培养基中可形成正常试管苗．再生植株在２５℃—３２℃温室中能正常开花结实．种子具生活力．
The present study forcused on the hybrid embryo rescue and plant reseneration of-interspecific hybridization between the cultivar of S. indicum and a wild species S. schizinizianum. In 9-13 days after pollination,the hybrid embryo could be rescued with high frequency, while over 13 days the hybrid embryo could be of abortion. MS mineral+ Nitcher organic nutrient + BA 0. 2 + GA30. 1 + CH600 + PVP0. 2% + Surcose5% was the desirable medium for the embryo in-vitro development. In-vitro-cultured embryo germinated in the MS medium and were then transfered to the MS +PP3330. 1 +BA 0. 2 for 2 weeks culture.The healthy seedlings regenerated in the MS+NAA 0. 5 and the plants developed in the pot under the 28℃-33℃ in greenhouse. The seeds of the regenerated plant were obtained.
出处
《中国油料》
CSCD
北大核心
1994年第1期33-35,共3页
基金
湖北省自然科学基金
关键词
芝麻
杂种胚营救
远缘杂种
Sesame cultivar
Wild species
Interspecific hybridization
Embryo rescue
Regeneration
