摘要
目的探讨反义核调节因子-κBp65(NFκBp65)在重症急性胰腺炎(SAP)时对肺组织的保护作用及机理。方法(1)固相亚磷酸三酯法人工合成反义NFκBp65寡核苷酸,并进行全硫代磷酸化修饰。(2)采用腹腔内直接注射反义NF-κBp65寡核苷酸于实验动物体内,应用免疫组织化学法检测其应用12、24h肺脏组织中NFκBp65的表达。结果(1)12、24h胰腺组织病理评分为:对照组(23.6±2.6)和(21.4±2.8)分,预防组(20.7±3.4)和(8.7±1.8)分,治疗组(9.20±2.3)和(14.7±1.9)分。同时各组间比较均有显著性差异(P<0.05)。(2)对照组12h和24h肺脏组织中NFκBp65表达的阳性细胞为(67.4±12.1)%和(59.7±13.4)%,预防组(49.3±9.2)%和(28.7±15.7)%,治疗组(42.9±1.9)%和(34.3±5.0)%。预防组、治疗组与对照组比较差异显著(P<0.01)。结论(1)STC诱导的SAP时肺脏组织中NFκB活性明显升高;(2)反义NF-κBp65对SAP时的肺脏组织损害不仅有保护作用而且有明显的治疗作用,其机理可能与改善SAP的病变程度有关。
Objective To investigate the protective effect of nuclear factor -κB p65 (NF-κB p65) gene on the lungs in the severe acute pancreatitis(SAP)rats. Methods (1) Rat NF-κB p65 antisense was synthesized by BioAsia.(2) The expression of NF-κB P 65 antisense in the lung tissue was detected by immunohistochemistry method.Results (1) Severity of pancreatitis in three groups was messured by microscope at the time points of 12 and 24hours.The pathologic scores of the pancreas at 12 and 24 hours after NF-κB p65 antisense injection were (23.6±2.6) and (21.4±2.8) in control group, (20.7±3.4) and (8.7±1.8) in prevention group, (9.20±2.3) and (14.7±1.9) in treating groups respectively(P<0.05). (2)The percentages of positive expression of NF-κB p65 of lung cells were (67.4±12.1)% and (59.7±13.4)% in control group,(49.3±9.2)% and (28.7±15.7)% in prevention group,(42.9±1.9)% and (30.3±5.0)% in treating groups respectively. There was a significant difference among three groups(P<0.01).Conclusions (1)NF-κB p65 plays an important role in the development of lung injury in severe acute pancreatitis. (2)NF-κB p65 antisense has a significant protective and therapeutic effects on the lungs in the acute pancreatitis rats.
出处
《江苏医药》
CAS
CSCD
北大核心
2005年第6期436-438,共3页
Jiangsu Medical Journal
基金
江苏省教育厅(00KJB320002)
