摘要
应用α－３２Ｐ－ｄＣＴＰ标记ＨＰＶ－１１及ＨＰＶ－１６ＤＮＡ为探针，以Ｓｌｏｔｂｌｏｔ及Ｓｏｕｔｈｅｒｎｂｌｏｔ两种核酸杂交技术，检测了取自青岛及北京两个地区的３７例喉鳞癌组织ＤＮＡ中ＨＰＶ－１１及ＨＰＶ－１６ＤＮＡ相关序列。结果表明，Ｓｌｏｔｂｌｏｔ杂交中，与ＨＰＶ－１１探针杂交阳性者占８６％（３２／３７），与ＨＰＶ－１６探针杂交阳性者占８１％（３０／３７）。在同一癌组织中，与两型探针同时呈现阳性者占８１％（３０／３７）。Ｓｏｕｔｈｅｒｎｂｌｏｔ杂交中，与所用探针ＤＮＡ的内切酶ＰｓｔⅠ酶切图谱比较，有４６％（１３／２８）与ＨＰＶ－１６ＤＮＡ的酶切图谱相同，可定为ＨＰＶ－１６型。当用ＨＰＶ－１１探针时无一例出现与ＨＰＶ－１１相同的图谱。提示喉癌的发生与ＨＰＶ－１６有关。
In this paper.α-32P-dCTP-labeled HPV-11 and HPV -16 DNA were used as probe,and slot and Southern blot hybridization techniques were used to detect HPV-11 and HPV-16 DNA related sequences in 37 laryngeal carcinoma tissues taken from patients in Qingdao and Beijng.Using the slot blot technique,86%(32/37)of the cases showed positive hybridization with HPV-11 probe,while 81%(30/37) showed positive results with HPV-16.Another 81%(30/37)showed positive hybridization with HPV-11 and HPV-16 simultaneously.Using Southern blot hybridization Pst digestion,the hybridization pattern corresponded to that of HPV-16 in 46;(13/28),and none corresponded to HPV-11.The results suggest that HPV-16 may induce laryngeal carcinoma.
出处
《中国医学科学院学报》
CAS
CSCD
北大核心
1994年第2期109-115,共7页
Acta Academiae Medicinae Sinicae
关键词
人
乳头瘤病毒
喉肿瘤
呼吸道肿瘤
laryngeal carcinoma
HPV
slot blot
hybridization
southern blot hybridization
