摘要
用反相离子对高效液相色谱法对三磷酸腺苷二钠原料（以下简称ＡＴＰ）及４种制剂进行定性定量。色谱条件：流动相为１０％乙腈－水（含１５ｍｍｏｌＩＰＲ－Ａ）；紫外检测波长２５４ｎｍ；色谱柱为国产ＹＷＧ－Ｃ＿（１８）。线性范围０．４～２．０μｇ，相关系数γ＝０．９９９８。测定结果，ＡＴＰ原料、注射液、片剂、胶囊及注射用粉针回收率依次为１００．８％，９９．１１％，１０１．７％，９７．６２％及１０１．０％。本法具有快速灵敏，准确实用等特点。
Adenosine triphosphate(ATP)and its four preparations were determined qualitatiely and quantitatively by reversed-phase ion-pair HPLC. Analvtical conditions included mobilephase:acetonitrile-water(1:9)containing 15mmol IPR-A;detecter wavelength:254nm;column:YWG-C18,(4mm×250mm);linear range:0.4～2.0μg;relative coefficient r=0.9998.The recov-eries of ATP raw rnaterial,injection,tablet,capsule and powder for injection were 100.8%, 99. 11%,101.7%,97.62%and 101.0%respectively.
出处
《中国药学杂志》
CSCD
北大核心
1994年第2期108-111,共4页
Chinese Pharmaceutical Journal
