摘要
用ＨＰＬＣ法分离测定牛黄解毒片中大黄素的含量。用正交设计法考察了甲醇浓度、流动相ｐＨ值、流速、柱温以及检测波长对方法耐用性的影响。色谱柱：Ｓｐｈｅｒｉｓｏｒｂ Ｃ（１８）（２５０ｍｍ×４．６ｍｍ，５μｍ），流动相：甲醇－水（Ｈ３ＰＯ４调ｐＨ为３．５）７８：２２，检测波长ＵＶ２５４ｎｍ，流速１ｍｌ／ｍｉｎ，大黄素的回收率为９８．２％，相对标准偏差ＲＳＤ＜２％。
The content of emedin in Niuhuang Jiedu Pian was determined by HPLC.A 10μl portion was injected into a column (250 mm ×4.6 mm) of Spherisorb C(18)(5μm), with CH3OH-H2O(pH 3. 5)78:22 asmobile phase(1.0 ml/min)and UV detection at 254 nm, at 2 0℃. The effect of methanol concentration, pH of mobile phase, flow rate, column temperature and detection on the method ruggeddness was evaluated .The variation of methonal concentration caused the main effect.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
1994年第6期335-338,共4页
Journal of China Pharmaceutical University
关键词
大黄素
牛黄解毒片
高效液相色谱
HPLC
Emodin
Niuhuang Jiedu Pian
Method ruggeddness
