摘要
５株已适应鸡胚细胞的ＡＶＡＶ在Ｖｅｒｏ细胞上传２～５代后各毒株均能产生明显的ＣＰＥ和较高的病毒滴度，而２株非鸡胚细胞适应株（分别为鸡胚毒和野外组织毒）在Ｖｅｒｏ细胞上盲传７代，仍无明显的ＣＰＥ出现。这一结果初步表明Ｖｅｒｏ细胞能用于已适应鸡胚细胞的毒株的增殖。而不能用于病毒的分离。吸附时间与冻融次数明显影响病毒的适应进程，以吸附１小时、冻融２次以上为佳。ＡＶＡＶＪＮ－１株能在Ｖｅｒｏ细胞上产生蚀斑，大小为２．７～４．４ｍｍ，出斑时间为接种后４～５天，Ｖｅｒｏ细胞用于中和试验，测得Ｓ（１１３３）株免疫鸡血清的小和效价为１：８０。
Abstract Five strains of AVAV,all of which had adapted to avian embryonic cell cultures, produced CPE and had great virus yield in vero cells after 2 to 5 passages.However, even after seven blind passages,other two strains of AVAV,both of which hadn,t adapted to avian embryonic cell cultures,couldn,t produced and CPE.The r.,Uits indicated that vero cells were Suitable for the reproduction of AVAV,but unsuitable for the isolation of AVAV from field materials.The absorption time and frozen-thawed times affected the adaptation of AVAV significantly.Our suggestion was that an absorption time for 1 hour and more than two times of freezing-thawing was better.JN-1 Strains of AVAV produced 2.7 to 4.4mm plaques in vero Calls, which appeared at 1 to 5 days after infection.Vero cells gcould also be applied to the detection for neutralizing antibody.
