摘要
目的探讨原发性肾病综合征(PNS)患儿肾组织激活蛋白_1(AP_1)、转化生长因子_β1(TGF_β1)的表达与激素耐药、肾脏病理损害的相关性,以期阐明PNS激素耐药的可能机制。方法应用非生物素免疫组化ElivisionTMplus法检测48例PNS(SSNS8例,SRNS20例,SDNS20例)患儿肾组织中AP_1亚基c_Jun和TGF_β1的表达水平,用计分法半定量评价肾脏的病理损害程度。结果①肾小球内和肾小管间质内AP_1和TGF_β1的表达,均为SRNS>SDNS>SSNS(P<0.01)。②SSNS、SDNS、SRNS3组肾小球的病理损害分数分别为4.25±1.49,5.25±1.65,8.10±2.57(SRNS与SDRS比较P<0.01,SDRS与SSNS比较P>0.05);SSNS、SDNS、SRNS3组肾小管间质的病理损害分数分别为4.13±0.99,6.90±1.55,11.10±2.94(P<0.01)。③肾组织中AP_1和TGF_β1的表达与肾小球和肾小管间质病理损害的程度呈正相关(相关系数分别为:0.463,0.352;0.547,0.646,P均<0.05)。结论SRNS患儿肾组织中AP_1和TGF_β1的表达增强,并与肾脏病理损害程度密切相关。
Objectives To explore the synthesis of AP-1and TGF-β 1 in renal biopsy tissue of patients with primary nephrotic syndrome(PNS),its correlation with both of the response for steroid therapy and the renal pathological lesions in order to demonstrate the possible mechanism of steroids-resistance. Methods Forty-eight PNS cases were divided into3groups consisting of8cases as steroid sensitive type(SSNS),20as steroid resistance type(SRNS)and20as steroid dependent type(SDNS),respectively.Three normal kidneys were as control group.Renal biopsy was performed in all cases.The synthesis of AP-1subunit c-Jun and TGF-β 1 were detected by ElivisionTM plus immunohistochemistry and renal histopathological lesion was evaluated by the semi- quantitative scores.Results(1)The synthesis of AP-1and TGF-β1in glomerulus and tubulointerstitium in SRNS group was most intense among three groups,and followed by SDNS group and SSNS group(P<0.01).(2)The scores of glomerular pathological lesion were4.25±1.49in SSNS group,5.25±1.65in SDRS group and8.10±2.57in SRNS group,respectively(SRNS vs SDRS,P<0.01;SDRS vs SSNS,P>0.05);the scores of tubulointerstitial pathological lesion were4.13±0.99in SSNS group,6.90±1.55in SDRS group and11.10±2.94in SRNS group,respectively(P<0.01).(3)The synthesis of renal AP-1and TGF-β 1 correlated with the severity of the glomerular and tubulointerstitial lesion(correlation coefficients:0.463,0.352;0.547,0.646).Conclusions Oversynthesis of AP-1and TGF-β 1 was observed in steroid-resistant children with PNS,and it correlated closely with renal pathological damage.
出处
《临床儿科杂志》
CAS
CSCD
北大核心
2005年第4期210-213,共4页
Journal of Clinical Pediatrics
