摘要
采用巢式RT-PCR(NT-PCR)检测HCVELISA阳性和阴性的全血标本,并与逆转录PCR(RT-PCR)的检测结果相比较。结果表明,采用巢式RT-PCR检测160例HCVELISA阳性标本HCVRNA的检出率为96.3±1.44,60例HCVELISA阴性标本HCVRNA的检出率为8.3±3.30;采用RT-PCR检测的检出率分别为58.8±5.08和1.7±6.70。结果提示,巢式RT-PCR是一种简便有效的提高RNA检测灵敏度的方法,适于临床血样中丙型肝炎病毒的RNA检测。
HCV-positive and HCV-negative blood samples(detected by ELISA) were detected with NT-PCR and RT-PCR. The results showed that the HCV RNA positive rates of 160 HCV-positive blood samples and 60 HCV-negative blood samples(detected by ELISA) were 96.3±1.44 and 8.3±3.30 when they were detected by NT-PCR, while the positive rate were 58.8±5.08 and 1.7±6.70 when they were detected by RT-PCR. The results imply that NT-PCR is sensitive method and can be applied in the detection of HCV in blood.
出处
《生物技术通讯》
CAS
2005年第3期280-281,共2页
Letters in Biotechnology
