摘要
应用纯化的Ｄ１１＾４株抗马立克氏病病毒中和性单克隆抗体，以ＳＰＡ－ＩｇＧ法和脂质体－ＩｇＧ法制成免疫原，分别免疫ＢＡＬＢ／ｃ小鼠，应用Ｂ淋巴细胞杂交瘤技术进行细胞融合，以两步ＥＬＩＳＡ筛选法获得了３株抗独特型单克隆抗体，分别命名为１Ｂ８、１０Ｃ１和３Ｄ５，其Ｉｇ亚类分别为ＩｇＧ１，ＩｇＧ１和ＩｇＧ２ｂ，染色体数在９２－１０２之间，在捕获阻断ＥＬＩＳＡ中。
Three anti-idiotypic antibodies (McAb,) named 1B810C1 and 3D5 were developed by fusion of mouse myeloma cell line NS-1 and spleen cells of BALB/c mice immunized with immunogens prepared from purified D114 neutralizing monoclonal antibodies to MDV and SPA or liposome respectively. The antibodies secreted by these hy-bridomas belong to IgG1 and IgG2 respectively. Binding activity of MDV antigen and McAb1 was specifically blocked by the McAb2 from mouse ascitic fluid in catcd-blockingELISA, and the blocking rate was 2. 66% to 76. 4% in the 1 :20 to 1:80 000 diluted McAb2 samples. The purified McAb2 which were emulsified with an equal volume of oil adjuvant were injected into 20-day-old chickens for three times and the serum samples were taken 10 days after final immunization and were tested for the presence of antibodies to MDV antigens. The results showed that the specific antibodies were present in the serum samples of immunized chickens. It was suggested that the configulafion of the variable regions of the McAb2s may be similar to those of antigenic determinant of MDV which combine with McAb1. The McAb2s will be useful in the research on monoclonal anti-idiotypic vaccine for Marek's disease.
出处
《中国兽医学报》
CAS
CSCD
1994年第3期223-227,共5页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目
关键词
单克隆抗体
马立克氏病
病毒
鸡病
monoclonal anti-idiotypic antibody
Marek's disease virus (MDV)
cell fusion
chicken
