摘要
本研究对水稻白叶枯病菌与水稻悬浮细胞非亲和互作中蛋白类激发子进行了分离纯化和鉴定。白叶枯病菌JXOⅤ与水稻IRBB4和IR24悬浮细胞互作 36h后的上清液,经Q Sepharose阴离子交换层析柱分离,对分离的各组分进行抗病性诱导测定,结果表明JXOⅤ与IRBB4非亲和互作的上清液中存在蛋白类激发子。有活性的蛋白组分经阴离子交换层析柱Mono Q进一步纯化后,SDS PAGE分析鉴定出 2个具激发活性的蛋白,其分子量分别为 17. 2kD和 49. 2kD,等电点分别为 5. 8和 6. 2。利用上述激发子处理水稻能减少病斑长度并诱导水稻防卫酶活性的增加。
In this study two proteinous elicitors in extracellular supernatant during interaction between Xanthomonas oryzae pv. oryzae JXOⅤ and suspension-cultured incompatible rice IRBB4 cells were isolated and identified. Proteins in extracellular supernatant collected 36 h after the interaction between JXOⅤ and incompatible rice IRBB4 suspension cells or compatible rice IR24 suspension cells were fractioned by HPLC using an anion exchange column Q-Sepharose. Two fractions obtained in incompatible interaction showed elicitor effect for that length of leaf lesion on rice IR24 inoculated JXOⅤ observably decreased after treatment with substance in that fractions. Further purification by HPLC using anion exchange column Mono-Q and preparative PAGE revealed 2 elicitors with molecular mass 17.2 kD and 49.2 kD,and pI 5.8 and 6.2. Treatment with these eli- citors at the concentration of 100 μg/mL before inoculation of JXOⅤ not only decreased leaf lesion,but also increased the activity of defence enzymes PAL and POD in leaves of compatible rice IR24.
出处
《植物病理学报》
CAS
CSCD
北大核心
2005年第1期55-59,共5页
Acta Phytopathologica Sinica
基金
国家自然科学基金项目(39900096)
国家"八六三"高技术研究发展计划(2001AA246013)
江苏省应用基础项目(BJ99032)
