摘要
以钙调素拮抗剂三氟拉嗪（ＴＦＰ）和环抱素Ａ（ＣｓＡ）为对照，观察抗钙调素（抗ＣａＭ）抗体对绵羊红细胞（ＳＲＢＣ）免疫的小鼠脾细胞及分泌抗巨细胞病毒（抗ＣＭＶ）单克隆抗体的杂交瘤细胞抗体分泌功能的影响。发现ＴＦＰ（０．１～１００μｍｏ／Ｌ）、ｃｓＡ（０．１～１００μ／Ｌ）明显抑制小鼠脾细胞与杂交瘤细胞分泌鼠Ｉｇ的功能，且此抑制作用随ＴＦＰ、ＣｓＡ剂量的增大而增强；与此不同，人和兔抗ＣａＭ对上述细胞分泌Ｉｇ有明显促进作用。
sing calmodulin(CaM)antagonist
including trifluoperazine(TFP ) and cyclosporin A(CsA)as drug
controls,we observed the effects of anticalmodulin
antibody(Anti-CaM)on theantibody secreting functions of the murine
apleen cells immunized with sheep red blcod cell(SR-BC)and the mouse
myeloma cells secreting anti-cyomegalo virus monoclonal antibody. The
re-sults showed that TFP(0.1～100μmol/L)and CsA(0.1～100μ
mol/L)significantly inhibitedthe Ig secreting function of the murine
spleen cells and the mouse myeloma cells and the inhibi-tion effects
were dose-dependent.In contrast to TFP and CsA,human or rabbit
anti-CaM en-hanced signifeantly function of these Ig secreting cells.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1994年第3期161-165,共5页
Chinese Journal of Immunology
基金
国家自然科学基金
关键词
抗体
抗体产生细胞
调钙蛋白
Cain1odulin Anti-CaM antibody Immunoglobulin-secreting cells
Trifluoperazine Cyclosporin A
