摘要
作者制备兔抗牛IgG酶结合物，以提供ELISA法准确鉴定蚊胃血中的牛血，检测牛、马、羊、鸡、猪、兔、鼠7种动物及人血清。证明酶结合物具有很高的特异性及敏感性，数十次ELISA测定结果表明，牛血清稀释度为1:100时，OD值在1.2～1.9之间，平均值为1.42，其它6种动物及人血清则在0～0.08之间，平均值0.01～0.06。此稀释度不但在酶联免疫检测仪中显示出明显的特异性，而且用肉眼也很容易识别和判断。
A enzyme-linked immunosorbent assay (ELISA）test was studied for detection of blood-meals in haematophagus mosquitoes.The conjugates of rabbit antibovine IgG were tested on serums of bovine,horse,goat,chicken,pig,rabbit ,mice and human;and more than the replicates of tests showed that the conjugates were proved both sensitive and specific.The conjugates were used at dilution of 1:100 and showed an average OD value of 1.42 while the serums from the six different animals showed an average OD value of only 0.01-0.06.The results indicate that the dilutions of 1:100 are not only high specific to detect with ELISA reader,but also easy to read by the naked eye.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
1994年第3期164-167,共4页
Chinese Journal of Vector Biology and Control
