摘要
目的研究含药物紫杉醇可降解支架材料——乙交酯/丙交酯共聚物(PLLGA)对人脐动脉平滑肌细胞(SMC)作用,检测此药物降解材料是否是血管内支架的理想材料。方法选取人脐动脉原代平滑肌细胞植块培养,得到传代细胞。然后与含不同紫杉醇药物浓度的降解材料一起培养,显微镜下观察降解材料远近处细胞形态发展的状态,观察金属支架组、Ⅰ组不含紫杉醇只含PLLGA对照组,Ⅱ～Ⅳ组分别含1、2、3μg紫杉醇PLLGA可降解材料对细胞生长的影响,并进行计数统计。结果降解材料对照组及金属支架组对平滑肌细胞生长无影响,含1、2、3μg紫杉醇的降解材料组细胞生长状态不佳。统计分析可见,72h,对照组、金属支架组与各用药组有显著统计学意义(P<0.01),但24h无统计学意义(P>0.05)。结论此紫杉醇降解材料(PLLGA)可作为抑制平滑肌细胞生长的血管内支架材料。
Objective To study the effect of paclitaxel containing degradable material (PLLA/PGA) on human umbilical arterial smooth muscle cells and detect whether it is an ideal material for intravascular stents. Methods The primary culture implant of human umbilical arterial smooth muscle cells was adopted.Then the secondary culture cells were obtained and cultured with degradable materials containing with different concentration of paclitaxel. The morphological development patterns of cells around degradable material were observed under microscope. The effects on cell growth in metal stent group,PLLGA containing only group,groups containing 1,2,3 μg paclitaxel PLLGA degradable materials were observed and statistically analyzed. Results In both degradable material control group and metal stent group,the growth of smooth muscle cell was not affected,but the cells in paclitaxel containing material group grew badly. Through statistical analysis the difference was significent (P < 0.01) betweent control group,metal stent group and drug group after 72 hours,but it was not obvious in 24 hours(P > 0.05). Conclusion The paclitaxel containing degradable material can be used in intravascular stent for inhibiling of smooth muscle cell growth.
出处
《生物医学工程与临床》
CAS
2005年第3期125-127,F002,共4页
Biomedical Engineering and Clinical Medicine
基金
2000年卫生部科技专项(2000)84号
吉林省科技厅赞助课题(20030051-4)
