摘要
选用12只雄性成年东北细毛羊,按随机区组试验设计,分别饲喂4个水平(分别为0, 100, 200, 300mg/kg)丝兰皂甙,研究了不同水平丝兰皂甙对绵羊瘤胃内纤维素酶、总脱氢酶活性及原虫数的影响。分别于试验当天及第7, 14,21, 28d采食后2h取瘤胃液,测定各种酶活及原虫数目,试验结果表明,随着丝兰皂甙添加水平的升高,脱氢酶活性有上升的趋势,但各组差异不显著(P】 0. 05);纤维素酶活性随着丝兰皂甙添加水平的升高,表现出上升的趋势,其中300mg/kg组同对照组、100mg/kg组相比达显著水平(P【 0. 05);各处理组内部原虫数随天数的增加变化无明显规律,但各处理组间有随着丝兰皂甙添加水平的升高,平均原虫数表现出下降的趋势, 300mg/kg组显著低于对照组和100mg/kg组(P【 0. 05),而同200mg/kg组差异不显著(P】 0. 05)。
In an experiment with complete randomized block design, 12 male adult local sheep were divided into 4 groups and administered sarsa-saponine at 0, 100, 200 and 300 mg/kg, respectively. Two hours after feeding, on 0, 7, 14, 21 and 28 days, samples of the rumen liquid were taken and the activities of cellulase and total dehydrogenase were determined and protozoa number was recorded. With the increase in sarsa-saponine level, dehydrogenase activity rose with no significant difference among the treatment groups (P>0.05), and cellulase activity also showed an ascending trend, the 300 and 100 mg/kg groups being significantly different from the control group (P<0.05). The variation in protozoa number showed no distinct pattern with sarsa-saponine levels, though it dropped with the increase in sarsa-saponine rate, generally. Protozoa number of the 300 mg/kg group was significantly smaller that that of the control and the 100 mg/kg group, but was non-significantly different from the 100 mg/kg group.
出处
《西南农业大学学报（自然科学版）》
CSCD
北大核心
2005年第2期214-218,共5页
Journal of Southwest Agricultural University
基金
中国科学院重点资助项目(161233)
关键词
丝兰皂甙
绵羊
原虫
瘤胃酶活
sarsa-saponine
sheep
protozoa
rumen enzyme activity
