摘要
目的探讨电离辐射诱导犬胆管成纤维细胞凋亡及防治胆管损伤后狭窄关系。方法将103Pd放射性支架,放射性活度为125×104kBq,和普通支架分别植入犬肝外胆管内,取出胆管标本,用DNA凝胶电泳检测成纤维细胞凋亡和透射电镜观察凋亡细胞形态和DNA的变化;并用计算机图像检测系统检测两组胆管腔面积。结果103Pd放射性支架明显抑制犬胆管组织中增生性瘢痕成纤维细胞生长,并出现典型的细胞凋亡特征,即出现凋亡小体、DNA梯状条带等。而且,103Pd放射性支架犬肝外胆管无明显狭窄,普通支架组犬胆管未出现成纤维细胞凋亡,胆管有明显狭窄。结论103Pd放射性支架诱导增生性瘢痕成纤维细胞凋亡是防治胆管损伤后由于增生瘢痕导致胆管狭窄的重要机理之一。
Objective To investigate the role of γ-ray-induced fibroblast apoptosis in the inhibition of biliary duct hypertrophic scar formation in dogs. Methods γ-radiation-induced apoptotic fibroblast cells were analysed by using transmission electron microscopy and DNA from frozen biliary duct tissue was extracted with phenol chloroform.DNA ladder profile after extraction of RNA was observed,and apoptosis cells in paraffineem-bedded biliary duct tissue sections were examined using immuno-histochemical method.Dog biliary duct cross-sections were stained with hematoxylin-erosin,Masson's trichrome,and Verhoeff-van Giesen stains.Muscle formation area,lumen circumference,and stenosis degree were determined by a computer-assisted image analysis system. Results ~ ~103 Pd radioactive stent significantly inhibited fibroblast proliferation.The features of fibroblast apoptosis (e.g.apoptic bodies,DNA ladder band) could be seen in the ~ ~103 Pd radioactive stent group.The fibroblast apoptotic rate was significantly increased in the ~ ~103 Pd radioactive stent group than in the control group(P<0.01).Comparing with the control group,the ~ ~103 Pd radioactive stent significantly reduced biliary muscular formation. Conclusion ~ ~103 Pd radioactive stent could have the effect of inhibiting the proliferation of scar-forming fibroblast,and thus could be used for treatment and (or) prevention of hypertrophic scar formation in biliary duct.;
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
2005年第2期118-120,共3页
Chinese Journal of Radiological Medicine and Protection
