摘要
对以胰蛋白酶为催化剂,琥珀明胶为底物的酶解反应体系进行了考察。结果表明,胰蛋白酶不仅可以切断琥珀明胶主链上的肽键,对琥珀酸分子上羧基和侧链氨基所形成的酰胺键也具有反应活性,导致降解产物的酰化度下降。研究了胰蛋白酶降解琥珀明胶的反应中,底物质量浓度、反应时间、温度、pH值和酶用量等条件对该反应的影响规律。找出了胰蛋白酶催化水解琥珀明胶的最适反应条件为:反应温度T=40℃,底物质量浓度约为100g L,体系pH=8 5,酶和底物质量比≥0 005。
In this paper,the enzymatic degradation of succinylated gelatin with trypsin was studied.It was proved that in the degradation process of succinylated gelatin,trypsin not only can cut off the peptide linkage in the principle chain,but also have reactive activity to amido link chaining the succinic acid group and amino,which lead to the decrease of acylation degrees of degradation products.The influences of the concentration of the succinylated gelatin,reaction time,temperature,pH value, and amount of enzyme on the hydrolysis process of succinylated gelatin by trypsin were investigated.The optimum conditions for this degradation system were:(T=40 ℃),mass concentration of substrate≈100 g/L,pH=8.5,the mass ratio of the enzyme to substrate≥0.005。
出处
《化学工业与工程》
CAS
2005年第3期206-210,共5页
Chemical Industry and Engineering
关键词
胰蛋白酶
降解
琥珀明胶
酰化度
trypsin
degradation
succinylated gelatin
acylation degree
