摘要
目的:研究小檗碱对人胃癌BGC- 82 3细胞诱导凋亡的作用。方法:应用荧光显微镜、透射电镜、流式细胞仪和TUNEL染色研究小檗碱对人胃癌BGC- 82 3细胞诱导凋亡的作用。结果:小檗碱作用人胃癌BGC -82 3细胞后,可看到较为典型的细胞凋亡的形态学变化,细胞核固缩、染色质凝集成新月状紧贴于核膜周边,核碎裂、染色质片断化、凋亡小体形成等。流式细胞仪上出现典型的亚二倍体凋亡峰。TUNEL染色显示,随小檗碱浓度增加和作用时间延长,细胞凋亡率增加。结论:小檗碱对人胃癌BGC- 82 3细胞有诱导凋亡作用。
Objective: To study the effects of berberine on human gastric carcinoma BGC-823 cell in induction of apoptosis. Methods: The apoptosis of human gastric carcinoma BGC-823 cell was studied by fluorescent microscopy, electronic microscopy, flow cytometry and TUNEL method after treatment with berberine. Results: After exposure to berberine, the human gastric carcinoma BGC-823 cell presented some morphologic features of apoptosis, including cell shrinkage, nuclear condensation, DNA fragmentation and apoptotic bodies formation. Some typical subdiploid peaks before G0/G1 phase were observed through flow cytometry. Along with berberine increased and prolonged, the apoptosis of human gastric carcinoma BGC-823 cell was raised after treatment with berberine. Conclusions: Berberine was able to induce the apoptosis in human gastric carcinoma BGC-823 cell.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2005年第1期16-19,共4页
Pharmacology and Clinics of Chinese Materia Medica
