摘要
目的:建立HPLC测定枳壳中新橙皮苷含量的方法。方法:选用色谱柱为Hypersil C18(250 mm×4.6 mm,5 μm); 流动相为乙腈0.5%磷酸水(pH=3)(19:81);检测波长283 nm;流速1.0 ml/min;柱温25℃。结果:新橙皮苷进样量在0.155-0.930μg范围内与峰面积呈良好线性关系(r=0.999 1,n=6);新橙皮苷的加样回收率103.12%(RSD= 1.22%)。结论:该法简便、准确、可靠,可用于枳壳药材的质量控制。
Objective: To establish a RP - HPLC method for determination of neohesperidin in Fructus Aurantn. Methods: The samples were extracted with methyl and the chromatographic conditions were as follow: Column: Hypersil C18(250 mm × 4. 6 mm, 5 μm). Mobile phase: acetonitrile-0. 5%H3PO4(volume ratio of 19 : 81). Detected at 283 nm. Flow rate: 1. 0 ml/mm . The column temperature : 25℃. Results:The calibration curves were linear in the range of 0. 155-0. 930 μg for neohespendm (r=0. 999 l,n = 6). The average recovery of nephesoeridin was 103. 12%(RSD=1. 22% ).Conclusion:This method is simple, accurate and reliable for the determination of neohesperidin in Fructus Aurantii, and can be used for the quality control of Fructus Anrantu.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2005年第4期280-281,共2页
Lishizhen Medicine and Materia Medica Research
基金
国家科技部2002年"中药材标准及其相关临床疗效评价标准"资助项目(No.ZTS-334-17)
关键词
枳壳
新橙皮苷
高效液相色谱法
Fructus Aurantii
Neohesperidin determination
RP-HPLC
