摘要
目的:探讨磨损微粒引起假体周围骨溶解过程中的骨保护能力,为人工关节假体松动的机制及预防研究打下基础。方法:M G-63细胞在无酚红的M EM培养基中单层培养,采用不同浓度细胞可吞噬钛微粒(粒径小于3μm对其进行24h干预或采用0.05%钛微粒干预0h、12h、24h、36h及48h,半定量R T-PC R观察其对护骨素)(OPG基因表达的影响。结果:随着钛微粒浓度及刺激时间逐渐增加,O PG m R N A表达上调逐渐增加。结论:在假体)松动病理过程中,骨保护能力增加,虽然其增加程度不及骨溶解,但为干预假体松动提供了基础。
Objective To investigate osteo protection induced by wear particles during the periprosthetic osteolysis in order to find out the mechanism of prosthetic aseptic loosening and found a basis for the prophylaxis of prosthetic aseptic loosening. Methods MG 63 cells were cultured as monolayers in phenol free Eagles minmum essential medium (MEM) and treated with different doses of phagocytosable titanium particles (less than 3 μm in diameter) for 24 hours and with 0.05%titanium particles for 0,12,24,36 and 48 hours, respectively. A semi quantitative RT PCR was taken to observe the osteoprotegerin(OPG) gene expression. Results OPG mRNA expression was up regulated gradually in a dose and time dependent manner with a certain range. Conclusion Osteo protecton increases during the pathogenesis of prosthetic loosening. Although the increasing extent of osteo protecton is less than that of osteolysis, it provides the foundation for intervening in the procedure of the aseptic loosening. <
出处
《实用医学杂志》
CAS
2005年第8期776-778,共3页
The Journal of Practical Medicine
