摘要
目的:探讨光波微波硝酸快速脱钙技术对骨及软组织抗原活性的影响。方法:用8%硝酸分别在单纯微波、单纯光波、光波微波组合Ⅰ和光波微波组合Ⅱ条件下脱钙,同时以室温脱钙作为对照;应用标记的链霉菌素卵白素生物素(LSAB)免疫组织化学(免疫组化)染色技术检测不同脱钙条件下心钠素(ANP)、5羟色胺(5HT)、S100、神经微丝蛋白(NF)、神经胶质酸性蛋白(GFAP)和波形蛋白(Vimentin)的表达,并对其染色结果进行图像分析和统计学处理。结果:8%硝酸室温脱钙时间最长,光波微波组合脱钙时间最短;微波组、光波组、光波微波组合Ⅰ组、Ⅱ组的免疫组化染色均明显比对照组好(P<0.01);光波微波组合Ⅰ组、Ⅱ组均明显好于单纯微波组(P<0.01)或单纯光波组(P<0.01);光波微波组合Ⅰ组与Ⅱ组,单纯光波组与单纯微波组之间的免疫组化染色无显著差异(P>0.05)。结论:光波微波硝酸脱钙所用时间比室温常规脱钙、单纯光波、微波脱钙时间明显缩短,免疫组化染色效果最好。
Objective:To investigate the effect of rapid decalcification by Lightwave/microwave nitric acid method on the antigenicity of osseous tissue.Methods:8% nitric acid (NA) solution was tested under four different decalcification condition: microwave group,Lightwave group,Lightwave/microwaveⅠ group and Lightwave/microwave Ⅱ group,respectively.Their decalcification efficiency was compared with that in control group at room temperature (20)℃ without microwave.The expressions of ANP,5-HT,S-100,NF,GFAP and Vimentin were detected by LSAB immunohistochemical method and image analysis under different decalcification conditions was conducted.Results:With NA as decalcifying agents,the decalcification time was the longest under the room temperature,but the shortest was achieved when combined with Lightwave/microwave method.Immunohistochemical staining was much better with microwave,Lightwave,lightwave/microwave Ⅰ and Ⅱmethods than control group (P<0.01);the immunohistochemical staining outcome between lightwave/microwave Ⅰand Ⅱ methods or between microwave and lightwave methods was not significantly different (P>0.05).Conclusions:With NA as decalcifying agents,the decalcification time with lightwave/microwave method was much shorter and the effect of immunohistochemical staining was much better than the routine method under the room temperature,microwave-only and lightwave-only methods.
出处
《华北国防医药》
2005年第2期84-86,F003,共4页
Medical Journal of Beijing Military Region
关键词
骨和骨组织
染色与标记
免疫组织化学
光波-微波
脱钙技术
对比研究
Bone & Osseous Tissue
Staining & labeling
Immunohistochemistry
Lightwave/microwave
Decalcification
Comparative study
