摘要
分别用粗糙脉孢菌(Neurosporacrassa)、紫红曲霉(Monascuspurpureus)、掷孢酵母(Sporobolomycesroseus)、深红酵母(Rhodotorularubra)、诺卡氏菌(Nocardiasp. )N89和游动放线菌(Actinoplanessp. )A05的细胞壁制成6种诱导子.当在玉米培养基中添加适量诱导子进行诱导培养时,青霉PT95菌株的菌核生物量和菌核中积累的类胡萝卜素含量有了显著的提高(P<0. 01),从而提高了PT95菌株的类胡萝卜素产率.其中, 4种真菌诱导子的效果明显好于另外2种放线菌诱导子,紫红曲霉诱导子的效果最好.当培养基中紫红曲霉诱导子的质量分数为100μg/g时,每百克玉米的类胡萝卜素产率达到14 446μg,比对照提高176%.除紫红曲霉诱导子外,其余5种诱导子都能显著提高总色素中的β-胡萝卜素百分含量(P<0. 01).
Six kinds of heat-released soluble cell-wall fragments (elicitors) were prepared from Neurospora crassa , Monascus purpureus , Sporolomyces roseus , Rhodotorula rubra , Nocardia sp. N89 and Actinoplanes sp. A05, respectively. When Penicillium sp. PT95 was grown on corn meal solid medium containing appropriate mass fraction (MF) of elicitors, both its sclerotia biomass and carotenoid content in sclerotia were enhanced significantly ( P <0.01). Among the elicitors tested, the elicitor from M. purpureus gave the highest carotenoid yield. When the MF of M. purpureus elicitor in medium was 100 μg/g, carotenoid yield per 100 g corn meal reached 14 446 μg, which was 1.76 times higher than that of control. Each of elicitors except that from M. purpureus could also significantly increase β -carotene fraction in pigment ( P <0.01). Tab 4, Ref 9
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2005年第2期208-210,共3页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学基金资助 (No. 30070021 )~~
关键词
青霉
诱导子
类胡萝卜素产率
菌核生物量
固态发酵
Penicillium sp.
elicitor
carotenoid yield
sclerotia biomass
solid-state fermentation
