摘要
目的探讨构建的重组丙型肝炎病毒(HCV)能否在易感细胞系中复制和表达。方法用人工构建的HCV感染易感细胞系7721,培养72h后利用RT-PCR检测HCV的RNA表达,并应用共聚焦激光扫描显微镜(CLSM)观察免疫荧光标记的HCV核心抗原和胞膜抗原E1在细胞中的表达。结果人工构建的HCV感染7721细胞72h后,RT-PCR检测细胞内可检出HCV正链及负链RNA,细胞培养上清中可检出HCV正链RNA,CLSM检测HCV核心抗原和E1抗原在细胞内的分布呈胞浆型。结论构建的HCV72h内可以在易感细胞系7721中复制和表达。
Objective To investigate whether recombinant hepatitis C virus (HCV) is capable of long-term replication in vivo in susceptible cells. Methods A human liver cell line 7721 was infected with recombinant HCV and after 72-hour incubation, the presence of HCV RNA in the cells and the supernatant were detected by reverse transcriptase (RT)-PCR. The expression of HCV antigen was detected by immunohistochemistry and observed by confocal laser scanning microscope. Results The plus and minus strands of HCV RNA were detected in the infected cells and the plus strand in the supernatant 72 h after infection. Expressions of HCV core antigens and E1 antigens were found within the cytoplasm. Conclusions The recombinant HCV can replicate in 7721 cell line in vitro and this HCV infection model can be useful for studying the mechanism of HCV infection and replication.
出处
《第一军医大学学报》
CSCD
北大核心
2005年第4期387-390,共4页
Journal of First Military Medical University
基金
国家863项目(2001AA215171)
广东省社会发展重大专项基金(A1090206)~~
