摘要
采用透射电镜术、电镜多糖细胞化学染色、细胞壁荧光染色以及香豆素抑制细胞壁再生等方法，对细叶黄芪（Ａｓｔｒａｇａｌｕｓｍ ｅｌｉｌｏｔｏｉｄｅｓ ｖａｒ．ｔｅｎｕｉｓ）叶肉原生质体细胞壁的再生及其化学特点进行了研究。结果表明，离体培养２４ 小时的原生质体表面产生一些突起小泡，有时可见少量纤维组分的形成。培养３ 天时这种纤维组分明显增多。至５ 天时可清楚看到再生壁是由纤维和颗粒构成。六亚甲四胺银染色证明它们都是由多糖组分组成的。另外，培养３６ 小时的原生质体有相互粘连的现象。电镜观察、荧光染色及香豆素处理的研究表明粘连与再生壁的形成有关。根据上述观察结果。
The techniques of transmission electron microscopy (TEM), electron microscopy (EM) cytochemical visualization of polysaccharide, cell wall flourescence labelling of cell wall and inhibition of wall formation by coumarin treatment were used to explore the cell wall regeneration and its chemical characteristics in mesophyll protoplasts of Astragalus melilotoides var.tenuis.The results showed that after 24 h in culture a number of protruding vesicles, as well as a small amount of fibrillar component were formed on the surface of protoplasts. On day 3, the amount of fibrils increased significantly. On day 5, regenerated primary wall composed of fibrils and granules were observed, in which polysacchaides were detected as result of the periodic acid silver methenamine reaction. In addition, after 36 h in culture, the protoplasts tended to coalesce, flourescence staining and coumarin treatment demonstrated that the protoplast adhesion was the result of cell wall formation. Based on these data, problems such as the structure of regenerated wall and its chemical nature, etc. were discussed.
关键词
叶肉
原生质体
细胞壁
再生
黄芪
Mesophyll protoplast
Cell wall regeneration
Cytochemistry of polysaccharide
Protoplast adhesion
Astragalus melilotoides var. tenuis
