摘要
用标记分析(signature analysis)法了解我国海南地区3年(1985—1987)来流行的登革2型(DEN-2)病毒株的抗原性变化。用3种单克隆抗体(McAb),包括黄病毒属特异、亚属特异和登革2型型特异,分析了8个DEN-2病毒流行株,并与标准株新几内亚B株进行比较。通过统计分析和微机处理,发现8株中有5株与株准株类似,有3株显示出明显差异。株记分析法为病毒抗原性分析提供了一个高度敏感的方法,并可用于监测一个地区病毒株群的变化及新株的引入。
In this paper, we try to define the extent of antigenic variation detween dengue-2 viruses from Hainan province, which had been isolated over a period of 3 years (1985—1987). The dengue-2 viruses were compared with the prototype New Guinea B strain and were subjected to antigen signature analysis. Eight strains of dengue-2 virus were analyzed by three monoclonal antibodies: flavivirus group, subcomplex dengue-2 type-specific reactive epitopes, over a range of antigen concentration.Five out of eight dengue-2 virus strains showed them to be antigenically homogeneous, and the remaining three strains showed them to be heterogeneous.Signature analysis provides a rapid and simple means to different strains derived from different sources, thus permit monitoring changes or introductions of hew dengue virus populations in certain geographic region.
出处
《微生物学报》
CAS
CSCD
北大核心
1989年第4期299-302,共4页
Acta Microbiologica Sinica
基金
国家自然科学基金
关键词
登革2型病毒
标记分析
单克隆抗体
Dengue-2 virus
Signature analysis
Monoclonal antibodies
